Download PDF by R. W. Fairbrother (Auth.): A Text-Book of Bacteriology

By R. W. Fairbrother (Auth.)

ISBN-10: 1483200310

ISBN-13: 9781483200316

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Additional resources for A Text-Book of Bacteriology

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When only one species of bacterium is present it is termed a pure culture. The inoculation of a second medium from a previous culture is termed a subculture. I t is important t h a t all cultures should be carefully labelled and the source of the material and date clearly indicated. The Incubation of Cultures. Inoculated media are placed in a thermostat at a temperature suitable for their growth. Thermostats or incubators working at 37° C. are necessary for the study of the parasitic bacteria. The incubators, water-jacketed and either gas or electrically heated, are regulated by capsules.

Pigment Production. Many bacteria are able to form a characteristic pigment ; these are termed chromogenic bacteria. The type of pigment varies considerably with different species and may be yellow, red, violet, green, etc. , Ps. , it is extracellular. The production of pigment is dependent to a considerable degree on environmental factors. Aerobic conditions are usually essential, but in the case of Sp. rubrum pigment-formation is most marked under reduced oxygen pressure. Pigment production is facilitated by growth at relatively low temperatures ; in many cases it is formed more readily on incubation at between 20°-30° C.

1. Viable Count. The number of living bacteria in a suspension is determined by plating a standard amount of serial decimal dilutions of the suspension. Dilutions in saline up to 1/107 or 1/108 of the original suspension are made ; a fresh pipette must be used for each bacterial transfer. c. of each dilution is placed in a Petri dish, melted agar, cooled to 50°-55° C, is added and the contents of the plate mixed by gentle agitation. After incubation at the optimum temperature for 24-48 hours, the total number of colonies on plates containing a countable number is obtained and an average made after the dilutions have been adjusted.

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A Text-Book of Bacteriology by R. W. Fairbrother (Auth.)

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