By Meral Beksac
This quantity is a compendium of state-of-the-art molecular equipment for the profitable transplantation of hematopoietic stem cells. The participants are world-renown leaders within the box. They describe promising instruments for stem mobile transplant examine versions, resembling in vivo bioluminescence imaging. They talk about HLA typing, PCR-SSP typing, and HLA antigens. This quantity is a useful resource for biochemists, molecular biologists, and clinicians.
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This quantity is a compendium of state of the art molecular tools for the profitable transplantation of hematopoietic stem cells. The participants are world-renown leaders within the box. They describe promising instruments for stem phone transplant study versions, reminiscent of in vivo bioluminescence imaging. They talk about HLA typing, PCR-SSP typing, and HLA antigens.
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Additional info for Bone Marrow and Stem Stell Transplantation (Methods in Molecular Medicine)
14. 15. 16. 17. 18. 19. 20. 33 means of pinhole SPECT in a TNBS colitis mouse model. Nucl. Med. Biol. 31, 93–101. Hildebrandt, I. J. and Gambhir, S. S. (2004) Molecular imaging applications for immunology. Clin. Immunol. 111, 210–224. , et al. (2004) Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J. Neurosci. Res. 76, 232–243. , et al. (2002) Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging.
11. 12. Duda et al. being able to rescue one or two mice per well, depending on the health of the cells in culture as assessed by light microscopy. Both calcium phosphate and liposome-mediated transfection methods have been used. This protocol describes a modified calcium phosphate-based method. Virus can be harvested again from the transfected packaging cells in 72 h. After collection, virus can be concentrated as previously listed. Discard the supernatant and store the viral pellet in the Eppendorf tube at –80°C.
Yang, P. , McConnell, M. , Pauly, J. , and Conolly, S. M. (2005) Positive contrast magnetic resonance imaging of cells labeled with magnetic nanoparticles. Magn. Reson. Med. 53, 999–1005. Kim, Y. , Gambhir, S. , and Witte, O. N. (2004) Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene. Mol. Imaging Biol. 6, 331–340. , and Germain, R. N. (2002) Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue.
Bone Marrow and Stem Stell Transplantation (Methods in Molecular Medicine) by Meral Beksac